Consensus statement on mass spectrometry-based proteomic analysis of cardiac tissue.
Lundby, Alicia , Van Eyk, Jennifer E , Mayr, Manuel , White, Melanie Y , Kirk, Jonathan A , Achter, Jonathan S , Fert-Bober, Justyna , Wierer, Michael , Mertins, Philipp , Lam, Maggie P Y , Humphrey, Sean J , Lau, Edward , Gramolini, Anthony O , Ge, Ying , Gundry, Rebekah L
Publication Details
- Journal: Nat Cardiovasc Res
- Published: May 2026
- Type: Journal Article
Abstract
Mass spectrometry-based cardiac proteomics provides direct molecular insight into
cardiac physiology and disease. While plasma proteomics has advanced biomarker discovery, the analysis of cardiac tissue is essential for mechanistic understanding and therapeutic target identification; however, proteomic investigation of cardiac tissue faces unique challenges, including limited sample availability, regional heterogeneity, variability in collection and processing, and inconsistent reporting practices that hinder reproducibility and data integration. Here, we provide a practical framework for designing and conducting mass spectrometry-based proteomic studies of cardiac tissue and primary cardiac cells. We outline best practices and key considerations for sample handling, experimental design, data acquisition, quality control and statistical analysis. This guideline aims to support cardiac researchers in generating robust and reproducible proteomics datasets that advance our understanding of cardiac biology in both physiological and pathological contexts.
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Added: June 09, 2026 | Updated: June 09, 2026