Publications

MiR-30 promotes fatty acid beta-oxidation and endothelial cell dysfunction and is a circulating biomarker of coronary microvascular dysfunction in pre-clinical models of diabetes.

Veitch, Shawn , Njock, Makon-Sebastien , Chandy, Mark , Siraj, M Ahsan , Chi, Lijun …

Journal Article Cardiovasc Diabetol Vol. 21 (1 ) pp. 31 (2022)

BACKGROUND: Type 2 diabetes (T2D) is associated with coronary microvascular dysfunction, which is thought to contribute to compromised diastolic function, ultimately culminating in heart failure with preserved ejection fraction (HFpEF). …

Serum response factor deletion 5 regulates phospholamban phosphorylation and calcium uptake.

Woulfe, Kathleen C , Jeffrey, Danielle A , Pires Da Silva, Julie , Wilson, Cortney …

Journal Article J Mol Cell Cardiol Vol. 159 pp. 28-37 (2021)

AIMS: Pediatric dilated cardiomyopathy (pDCM) is characterized by unique age-dependent molecular mechanisms that include myocellular responses to therapy. We previously showed that pDCM, but not adult DCM patients respond to …

Transcriptome features of striated muscle aging and predictability of protein level changes.

Han, Yu , Li, Lauren Z , Kastury, Nikhitha L , Thomas, Cody T , …

Journal Article Mol Omics Vol. 17 (5 ) pp. 796-808 (2021)

We performed total RNA sequencing and multi-omics analysis comparing skeletal muscle and cardiac muscle in young adult (4 months) vs. early aging (20 months) mice to examine the molecular mechanisms …

Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot.

Han, Yu , Thomas, Cody T , Wennersten, Sara A , Lau, Edward , Lam, …

Journal Article J Vis Exp (2021)

Mass spectrometry-based shotgun proteomics experiments require multiple sample preparation steps, including enzymatic protein digestion and clean-up, which can take up significant person-hours of bench labor and present a source of …

Method for selective ablation of undifferentiated human pluripotent stem cell populations for cell-based therapies.

Chour, Tony , Tian, Lei , Lau, Edward , Thomas, Dilip , Itzhaki, Ilanit , …

Journal Article JCI Insight Vol. 6 (7 ) (2021)

Human pluripotent stem cells (PSCs), which are composed of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), provide an opportunity to advance cardiac cell therapy-based clinical trials. However, …

Atlas of Exosomal microRNAs Secreted From Human iPSC-Derived Cardiac Cell Types.

Chandy, Mark , Rhee, June-Wha , Ozen, Mehmet O , Williams, Damon R , Pepic, …

Journal Article Circulation Vol. 142 (18 ) pp. 1794-1796 (2020)

Proteomic signatures of acute oxidative stress response to paraquat in the mouse heart.

Dostal, Vishantie , Wood, Silas D , Thomas, Cody T , Han, Yu , Lau, …

Journal Article Sci Rep Vol. 10 (1 ) pp. 18440 (2020)

The heart is sensitive to oxidative damage but a global view on how the cardiac proteome responds to oxidative stressors has yet to fully emerge. Using quantitative tandem mass spectrometry, …

High-throughput Preparation of DNA, RNA, and Protein from Cryopreserved Human iPSCs for Multi-omics Analysis.

Zhang, Jeffrey X , Lau, Edward , Paik, David T , Zhuge, Yan , Wu, …

Journal Article Curr Protoc Stem Cell Biol Vol. 54 (1 ) pp. e114 (2020)

We describe the procedure to isolate genomic DNA, RNA, and protein directly from cryopreserved induced pluripotent stem cell (iPSC) vials using commercially available solid-phase extraction kits, and we report the …

Modeling Secondary Iron Overload Cardiomyopathy with Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Rhee, June-Wha , Yi, Hyoju , Thomas, Dilip , Lam, Chi Keung , Belbachir, Nadjet …

Journal Article Cell Rep Vol. 32 (2 ) pp. 107886 (2020)

Excessive iron accumulation in the heart causes iron overload cardiomyopathy (IOC), which initially presents as diastolic dysfunction and arrhythmia but progresses to systolic dysfunction and end-stage heart failure when left …

De novo mutations across 1,465 diverse genomes reveal mutational insights and reductions in the Amish founder population.

Kessler, Michael D , Loesch, Douglas P , Perry, James A , Heard-Costa, Nancy L …

Journal Article Proc Natl Acad Sci U S A Vol. 117 (5 ) pp. 2560-2569 (2020)

De novo mutations (DNMs), or mutations that appear in an individual despite not being seen in their parents, are an important source of genetic variation whose impact is relevant to …

Splice-Junction-Based Mapping of Alternative Isoforms in the Human Proteome.

Lau, Edward , Han, Yu , Williams, Damon R , Thomas, Cody T , Shrestha, …

Journal Article Cell Rep Vol. 29 (11 ) pp. 3751-3765.e5 (2019)

The protein-level translational status and function of many alternative splicing events remain poorly understood. We use an RNA sequencing (RNA- seq)-guided proteomics method to identify protein alternative splicing isoforms in …

Activation of PDGF pathway links LMNA mutation to dilated cardiomyopathy.

Lee, Jaecheol , Termglinchan, Vittavat , Diecke, Sebastian , Itzhaki, Ilanit , Lam, Chi Keung …

Journal Article Nature Vol. 572 (7769 ) pp. 335-340 (2019)

Lamin A/C (LMNA) is one of the most frequently mutated genes associated with dilated cardiomyopathy (DCM). DCM related to mutations in LMNA is a common inherited cardiomyopathy that is associated …

A Premature Termination Codon Mutation in MYBPC3 Causes Hypertrophic Cardiomyopathy via Chronic Activation of Nonsense-Mediated Decay.

Seeger, Timon , Shrestha, Rajani , Lam, Chi Keung , Chen, Caressa , McKeithan, Wesley …

Journal Article Circulation Vol. 139 (6 ) pp. 799-811 (2019)

BACKGROUND: Hypertrophic cardiomyopathy (HCM) is frequently caused by mutations in myosin-binding protein C3 ( MYBPC3) resulting in a premature termination codon (PTC). The underlying mechanisms of how PTC mutations in …

Systems-Wide Approaches in Induced Pluripotent Stem Cell Models.

Lau, Edward , Paik, David T , Wu, Joseph C

Journal Article Annu Rev Pathol Vol. 14 pp. 395-419 (2019)

Human induced pluripotent stem cells (iPSCs) provide a renewable supply of patient-specific and tissue-specific cells for cellular and molecular studies of disease mechanisms. Combined with advances in various omics technologies, …

Identifying High-Priority Proteins Across the Human Diseasome Using Semantic Similarity.

Lau, Edward , Venkatraman, Vidya , Thomas, Cody T , Wu, Joseph C , Van …

Journal Article J Proteome Res Vol. 17 (12 ) pp. 4267-4278 (2018)

Identifying the genes and proteins associated with a biological process or disease is a central goal of the biomedical research enterprise. However, relatively few systematic approaches are available that provide …

Omics, Big Data, and Precision Medicine in Cardiovascular Sciences.

Lau, Edward , Wu, Joseph C

Journal Article Circ Res Vol. 122 (9 ) pp. 1165-1168 (2018)

Prolonged survival of transplanted stem cells after ischaemic injury via the slow release of pro-survival peptides from a collagen matrix.

Lee, Andrew S , Inayathullah, Mohammed , Lijkwan, Maarten A , Zhao, Xin , Sun, …

Journal Article Nat Biomed Eng Vol. 2 (2 ) pp. 104-113 (2018)

Stem-cell-based therapies hold considerable promise for regenerative medicine. However, acute donor-cell death within several weeks after cell delivery remains a critical hurdle for clinical translation. Co- transplantation of stem cells …

NextGen VOICES: Research resolutions.

Olmeta-Schult, Felicia , Segal, Lauren Massa , Tyner, Sam , Moon, Twila Alexandra , Chow, …

Journal Article Science Vol. 359 (6371 ) pp. 26-28 (2018)

Integrated omics dissection of proteome dynamics during cardiac remodeling.

Lau, Edward , Cao, Quan , Lam, Maggie P Y , Wang, Jie , Ng, …

Journal Article Nat Commun Vol. 9 (1 ) pp. 120 (2018)

Transcript abundance and protein abundance show modest correlation in many biological models, but how this impacts disease signature discovery in omics experiments is rarely explored. Here we report an integrated …

Cardiovascular proteomics in the era of big data: experimental and computational advances.

Lam, Maggie P Y , Lau, Edward , Ng, Dominic C M , Wang, Ding …

Review Article Clin Proteomics Vol. 13 pp. 23 (2016)

Proteomics plays an increasingly important role in our quest to understand cardiovascular biology. Fueled by analytical and computational advances in the past decade, proteomics applications can now go beyond merely …