Publications
Research output from the Lau Laboratory
2025
Longevity of cardiac and skeletal muscle proteins is dependent on tissue and subcellular compartmentation patterns.
Gugel, Jack , Currie, Jordan , Alamillo, Lorena , Flint, Jason , Kim, Keun-Young , …
Journal Article Cell Rep Vol. 45 (1 ) pp. 116768 (2025)
Myocytes are exceptionally long-lived cells that must maintain proteome integrity over decades while adjusting for changes in functional output and metabolic demand. We used in vivo stable isotope labeling combined …
SALVE: prediction of interorgan communication with transcriptome latent space representation.
Pavelka, Jay , Voong, Calvin K , Schaal, Peyton , Lam, Maggie P Y , …
Journal Article Am J Physiol Heart Circ Physiol Vol. 329 (6 ) pp. H1621-H1632 (2025)
Massive transcriptomics data allow gene relationships to be discovered from their correlated expression. We describe secretome association with latent variables between tissues (SALVE), a method to infer the associations between …
A ratiometric catalog of protein isoform shifts in the cardiac fetal gene program.
Han, Yu , Binti, Shaonil , Wennersten, Sara A , Pandi, Boomathi , Ng, Dominic …
Journal Article JCI Insight Vol. 10 (18 ) (2025)
Pathological cardiac remodeling is associated with the reactivation of fetal genes, yet the extent of the heart's fetal gene program and its impact on proteome compositions remain incompletely understood. Here, …
Protein Turnover Dynamics Analysis With Subcellular Spatial Resolution.
Alamillo, Lorena , Black, Alexander , Lam, Maggie P Y , Lau, Edward
Journal Article Bio Protoc Vol. 15 (15 ) pp. e5409 (2025)
Protein synthesis and degradation (i.e., turnover) forms an important part of protein homeostasis and has been implicated in many age- associated diseases. Different cellular locations, such as organelles and membraneless …
Deuterium labeling enables proteome-wide turnover kinetics analysis in cell culture.
Alamillo, Lorena , Ng, Dominic C M , Currie, Jordan , Black, Alexander , Pandi, …
Journal Article Cell Rep Methods Vol. 5 (7 ) pp. 101104 (2025)
Protein turnover is a critical component of gene expression regulation and cellular homeostasis, yet methods for measuring turnover rates that are scalable and applicable to different models are still needed. …
A Ratiometric Catalog of Protein Isoform Shifts in the Cardiac Fetal Gene Program.
Han, Yu , Binti, Shaonil , Wennersten, Sara A , Pandi, Boomathi , Ng, Dominic …
Journal Article bioRxiv (2025)
Pathological cardiac remodeling is associated with the reactivation of fetal genes, yet the extent of the heart's fetal gene program and its impact on proteome compositions remain incompletely understood. Here, …
Improved Method to Determine Protein Turnover Rates with Heavy Water Labeling by Mass Isotopomer Ratio Selection.
Currie, Jordan , Ng, Dominic C M , Pandi, Boomathi , Black, Alexander , Manda, …
Journal Article J Proteome Res Vol. 24 (4 ) pp. 1992-2005 (2025)
The synthesis and degradation rates of proteins form an essential component of gene expression control. Heavy water labeling has been used in conjunction with mass spectrometry to measure protein turnover …
Deuterium labeling enables proteome wide turnover kinetics analysis in cell culture.
Alamillo, Lorena , Ng, Dominic C M , Currie, Jordan , Black, Alexander , Pandi, …
Journal Article bioRxiv (2025)
The half-life of proteins is tightly regulated and underlies many cellular processes. It remains unclear the extent to which proteins are dynamically synthesized and degraded in different cell types and …
2024
Tissue Usage Preference and Intrinsically Disordered Region Remodeling of Alternative Splicing Derived Proteoforms in the Heart.
Pandi, Boomathi , Brenman, Stella , Black, Alexander , Ng, Dominic C M , Lau, …
Journal Article J Proteome Res Vol. 23 (8 ) pp. 3161-3173 (2024)
A computational analysis of mass spectrometry data was performed to uncover alternative splicing derived protein variants across chambers of the human heart. Evidence for 216 non-canonical isoforms was apparent in …
Improved determination of protein turnover rate with heavy water labeling by mass isotopomer ratio selection.
Currie, Jordan , Ng, Dominic C M , Pandi, Boomathi , Black, Alexander , Manda, …
Journal Article bioRxiv (2024)
The synthesis and degradation rates of proteins form an essential component of gene expression control. Heavy water labeling has been used in conjunction with mass spectrometry to measure protein turnover …
Proteomics applications in next generation induced pluripotent stem cell models.
Manda, Vyshnavi , Pavelka, Jay , Lau, Edward
Journal Article Expert Rev Proteomics Vol. 21 (4 ) pp. 217-228 (2024)
INTRODUCTION: Induced pluripotent stem (iPS) cell technology has transformed biomedical research. New opportunities now exist to create new organoids, microtissues, and body-on-a-chip systems for basic biology investigations and clinical translations. …
Simultaneous proteome localization and turnover analysis reveals spatiotemporal features of protein homeostasis disruptions.
Currie, Jordan , Manda, Vyshnavi , Robinson, Sean K , Lai, Celine , Agnihotri, Vertica …
Journal Article Nat Commun Vol. 15 (1 ) pp. 2207 (2024)
The spatial and temporal distributions of proteins are critical to protein function, but cannot be directly assessed by measuring protein bundance. Here we describe a mass spectrometry-based proteomics strategy, Simultaneous …
Simultaneous proteome localization and turnover analysis reveals spatiotemporal features of protein homeostasis disruptions.
Currie, Jordan , Manda, Vyshnavi , Robinson, Sean K , Lai, Celine , Agnihotri, Vertica …
Preprint bioRxiv (2024)
The functions of proteins depend on their spatial and temporal distributions, which are not directly measured by static protein abundance. Under endoplasmic reticulum (ER) stress, the unfolded protein response (UPR) …
2023
Tissue Usage Preference and Intrinsically Disordered Region Remodeling of Alternative Splicing Derived Proteoforms in the Heart.
Pandi, Boomathi , Brenman, Stella , Black, Alexander , Ng, Dominic C M , Lau, …
Preprint bioRxiv (2023)
A computational analysis of mass spectrometry data was performed to uncover alternative splicing derived protein variants across chambers of the human heart. Evidence for 216 non-canonical isoforms was apparent in …
2022
Defining the Roles of Cardiokines in Human Aging and Age-Associated Diseases.
Srivastava, Himangi , Pozzoli, Marina , Lau, Edward
Journal Article Front Aging Vol. 3 pp. 884321 (2022)
In recent years an expanding collection of heart-secreted signaling proteins have been discovered that play cellular communication roles in diverse pathophysiological processes. This minireview briefly discusses current evidence for the …
Protein prediction models support widespread post-transcriptional regulation of protein abundance by interacting partners.
Srivastava, Himangi , Lippincott, Michael J , Currie, Jordan , Canfield, Robert , Lam, Maggie …
Journal Article PLoS Comput Biol Vol. 18 (11 ) pp. e1010702 (2022)
Protein and mRNA levels correlate only moderately. The availability of proteogenomics data sets with protein and transcript measurements from matching samples is providing new opportunities to assess the degree to …
Sex-specific responses to slow progressive pressure overload in a large animal model of HFpEF.
Eaton, Deborah M , Berretta, Remus M , Lynch, Jacqueline E , Travers, Joshua G …
Journal Article Am J Physiol Heart Circ Physiol Vol. 323 (4 ) pp. H797-H817 (2022)
Approximately 50% of all heart failure (HF) diagnoses can be classified as HF with preserved ejection fraction (HFpEF). HFpEF is more prevalent in females compared with males, but the underlying …
Proteogenomics reveals sex-biased aging genes and coordinated splicing in cardiac aging.
Han, Yu , Wennersten, Sara A , Wright, Julianna M , Ludwig, R W , …
Journal Article Am J Physiol Heart Circ Physiol Vol. 323 (3 ) pp. H538-H558 (2022)
The risks of heart diseases are significantly modulated by age and sex, but how these factors influence baseline cardiac gene expression remains incompletely understood. Here, we used RNA sequencing and …
Harmonizing Labeling and Analytical Strategies to Obtain Protein Turnover Rates in Intact Adult Animals.
Hammond, Dean E , Simpson, Deborah M , Franco, Catarina , Wright Muelas, Marina , …
Journal Article Mol Cell Proteomics Vol. 21 (7 ) pp. 100252 (2022)
Changes in the abundance of individual proteins in the proteome can be elicited by modulation of protein synthesis (the rate of input of newly synthesized proteins into the protein pool) …
Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammation.
Wei, Tzu-Tang , Chandy, Mark , Nishiga, Masataka , Zhang, Angela , Kumar, Kaavya Krishna …
Journal Article Cell Vol. 185 (10 ) pp. 1676-1693.e23 (2022)
Epidemiological studies reveal that marijuana increases the risk of cardiovascular disease (CVD); however, little is known about the mechanism. Delta(9)-tetrahydrocannabinol (Delta(9)-THC), the psychoactive component of marijuana, binds to cannabinoid receptor …