Publications

Online combination of reversed-phase/reversed-phase and porous graphitic carbon liquid chromatography for multicomponent separation of proteomics and glycoproteomics samples.

Lam, Maggie P Y , Lau, Edward , Siu, S O , Ng, Dominic C …

Journal Article Electrophoresis Vol. 32 (21 ) pp. 2930-40 (2011)

In this paper, we describe an online combination of reversed- phase/reversed-phase (RP-RP) and porous graphitic carbon (PGC) liquid chromatography (LC) for multicomponent analysis of proteomics and glycoproteomics samples. The online …

Fully automatable two-dimensional reversed-phase capillary liquid chromatography with online tandem mass spectrometry for shotgun proteomics.

Siu, S O , Lam, Maggie P Y , Lau, Edward , Kong, Ricky P …

Journal Article Proteomics Vol. 11 (11 ) pp. 2308-19 (2011)

Herein, we describe the development of a fully automatable technology that features online coupling of high-pH RP separation with conventional low-pH RP separation in a two-dimensional capillary liquid chromatography (2-D …

Combinatorial use of offline SCX and online RP-RP liquid chromatography for iTRAQ-based quantitative proteomics applications.

Lau, Edward , Lam, Maggie P Y , Siu, S O , Kong, Ricky P …

Journal Article Mol Biosyst Vol. 7 (5 ) pp. 1399-408 (2011)

Extensive front-end separation is usually required for complex samples in bottom-up proteomics to alleviate the problem of peptide undersampling. Isobaric Tags for Relative and Absolute Quantification (iTRAQ)-based experiments have particularly …

N-linked glycoprotein analysis using dual-extraction ultrahigh- performance liquid chromatography and electrospray tandem mass spectrometry.

Siu, S O , Lam, Maggie P Y , Lau, Edward , Yeung, William S …

Journal Article Methods Mol Biol Vol. 600 pp. 133-43 (2010)

Although reverse-phase liquid chromatography (RP-LC) is a common technique for peptide separation in shotgun proteomics and glycoproteomics, it often provides unsatisfactory results for the analysis of glycopeptides and glycans. This …

Online coupling of reverse-phase and hydrophilic interaction liquid chromatography for protein and glycoprotein characterization.

Lam, Maggie P Y , Siu, S O , Lau, Edward , Mao, Xiuli , …

Journal Article Anal Bioanal Chem Vol. 398 (2 ) pp. 791-804 (2010)

We have developed a novel system for coupling reverse-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) online in a micro- flow scheme. In this approach, the inherent solvent incompatibility between …

OsNOA1/RIF1 is a functional homolog of AtNOA1/RIF1: implication for a highly conserved plant cGTPase essential for chloroplast function.

Liu, Hongjia , Lau, Edward , Lam, Maggie P Y , Chu, Hung , Li, …

Journal Article New Phytol Vol. 187 (1 ) pp. 83-105 (2010)

*The bacterial protein YqeH is a circularly permuted GTPase with homologs encoded by plant nuclear genomes. The rice homolog OsNOA1/RIF1 is encoded by the single-copy gene Os02g01440. OsNOA1/RIF1 is expressed …