Publications

Data-Driven Approach To Determine Popular Proteins for Targeted Proteomics Translation of Six Organ Systems.

Lam, Maggie P Y , Venkatraman, Vidya , Xing, Yi , Lau, Edward , Cao, …

Journal Article J Proteome Res Vol. 15 (11 ) pp. 4126-4134 (2016)

Amidst the proteomes of human tissues lie subsets of proteins that are closely involved in conserved pathophysiological processes. Much of biomedical research concerns interrogating disease signature proteins and defining their …

Connecting the Dots: From Big Data to Healthy Heart.

Lau, Edward , Watson, Karol E , Ping, Peipei

Journal Article Circulation Vol. 134 (5 ) pp. 362-4 (2016)

Identifying Low-Abundance Biomarkers: Aptamer-Based Proteomics Potentially Enables More Sensitive Detection in Cardiovascular Diseases.

Gramolini, Anthony , Lau, Edward , Liu, Peter P

Journal Article Circulation Vol. 134 (4 ) pp. 286-9 (2016)

A large dataset of protein dynamics in the mammalian heart proteome.

Lau, Edward , Cao, Quan , Ng, Dominic C M , Bleakley, Brian J , …

Journal Article Sci Data Vol. 3 pp. 160015 (2016)

Protein stability is a major regulatory principle of protein function and cellular homeostasis. Despite limited understanding on mechanisms, disruption of protein turnover is widely implicated in diverse pathologies from heart …

Label-Free Quantitative Proteomics Reveals Survival Mechanisms Developed by Hypertrophic Chondrocytes under ER Stress.

Kudelko, Mateusz , Chan, Cecilia W L , Sharma, Rakesh , Yao, Qing , Lau, …

Journal Article J Proteome Res Vol. 15 (1 ) pp. 86-99 (2016)

Emerging evidence implicates ER stress caused by unfolded mutant proteins in chondrocytes as the underlying pathology of chondrodysplasias. ER stress is triggered in hypertrophic chondrocytes (HCs) in a mouse model …

Prioritizing Proteomics Assay Development for Clinical Translation.

Lam, Maggie P Y , Venkatraman, Vidya , Cao, Quan , Wang, Ding , Dincer, …

Journal Article J Am Coll Cardiol Vol. 66 (2 ) pp. 202-4 (2015)

Spatial and temporal dynamics of the cardiac mitochondrial proteome.

Lau, Edward , Huang, Derrick , Cao, Quan , Dincer, T Umut , Black, Caitie …

Journal Article Expert Rev Proteomics Vol. 12 (2 ) pp. 133-46 (2015)

Mitochondrial proteins alter in their composition and quantity drastically through time and space in correspondence to changing energy demands and cellular signaling events. The integrity and permutations of this dynamism …

Mitochondrial protein turnover: methods to measure turnover rates on a large scale.

Chan, X'avia C Y , Black, Caitlin M , Lin, Amanda J , Ping, Peipei …

Journal Article J Mol Cell Cardiol Vol. 78 pp. 54-61 (2015)

Mitochondrial proteins carry out diverse cellular functions including ATP synthesis, ion homeostasis, cell death signaling, and fatty acid metabolism and biogenesis. Compromised mitochondrial quality control is implicated in various human …

Characterization of human plasma proteome dynamics using deuterium oxide.

Wang, Ding , Liem, David A , Lau, Edward , Ng, Dominic C M , …

Journal Article Proteomics Clin Appl Vol. 8 (7-8 ) pp. 610-9 (2014)

PURPOSE: High-throughput quantification of human protein turnover via in vivo administration of deuterium oxide ((2) H2 O) is a powerful new approach to examine potential disease mechanisms. Its immediate clinical …

Lysine ubiquitination and acetylation of human cardiac 20S proteasomes.

Zong, Nobel , Ping, Peipei , Lau, Edward , Choi, Howard Jh , Ng, Dominic …

Journal Article Proteomics Clin Appl Vol. 8 (7-8 ) pp. 590-594 (2014)

PURPOSE: Altered proteasome functions are associated with multiple cardiomyopathies. While the proteasome targets polyubiquitinated proteins for destruction, it itself is modifiable by ubiquitination. We aim to identify the exact ubiquitination …

Protein kinetic signatures of the remodeling heart following isoproterenol stimulation.

Lam, Maggie P Y , Wang, Ding , Lau, Edward , Liem, David A , …

Journal Article J Clin Invest Vol. 124 (4 ) pp. 1734-44 (2014)

Protein temporal dynamics play a critical role in time-dimensional pathophysiological processes, including the gradual cardiac remodeling that occurs in early-stage heart failure. Methods for quantitative assessments of protein kinetics are …

Characterization, design, and function of the mitochondrial proteome: from organs to organisms.

Lotz, Christopher , Lin, Amanda J , Black, Caitlin M , Zhang, Jun , Lau, …

Journal Article J Proteome Res Vol. 13 (2 ) pp. 433-46 (2014)

Mitochondria are a common energy source for organs and organisms; their diverse functions are specialized according to the unique phenotypes of their hosting environment. Perturbation of mitochondrial homeostasis accompanies significant …

Cyclophilin D and acetylation: a new link in cardiac signaling.

Lam, Maggie P Y , Lau, Edward , Liem, David A , Ping, Peipei

Journal Article Circ Res Vol. 113 (12 ) pp. 1268-9 (2013)

Response to Pomozi et al's research commentary.

Martin, Lisa J , Lau, Edward , Singh, Harpreet , Vergnes, Laurent , Tarling, Elizabeth …

Journal Article Circ Res Vol. 112 (11 ) pp. e152-3 (2013)

Site-specific quantitative analysis of cardiac mitochondrial protein phosphorylation.

Lam, Maggie P Y , Lau, Edward , Scruggs, Sarah B , Wang, Ding , …

Journal Article J Proteomics Vol. 81 pp. 15-23 (2013)

We report the development of a multiple-reaction monitoring (MRM) strategy specifically tailored to the detection and quantification of mitochondrial protein phosphorylation. We recently derived 68 MRM transitions specific to protein …

Metabolic labeling reveals proteome dynamics of mouse mitochondria.

Kim, Tae-Young , Wang, Ding , Kim, Allen K , Lau, Edward , Lin, Amanda …

Journal Article Mol Cell Proteomics Vol. 11 (12 ) pp. 1586-94 (2012)

Mitochondrial dysfunction is associated with many human diseases. Mitochondrial damage is exacerbated by inadequate protein quality control and often further contributes to pathogenesis. The maintenance of mitochondrial functions requires a …

An MRM-based workflow for quantifying cardiac mitochondrial protein phosphorylation in murine and human tissue.

Lam, Maggie P Y , Scruggs, Sarah B , Kim, Tae-Young , Zong, Chenggong , …

Journal Article J Proteomics Vol. 75 (15 ) pp. 4602-9 (2012)

The regulation of mitochondrial function is essential for cardiomyocyte adaptation to cellular stress. While it has long been understood that phosphorylation regulates flux through metabolic pathways, novel phosphorylation sites are …

ABCC6 localizes to the mitochondria-associated membrane.

Martin, Lisa J , Lau, Edward , Singh, Harpreet , Vergnes, Laurent , Tarling, Elizabeth …

Journal Article Circ Res Vol. 111 (5 ) pp. 516-20 (2012)

RATIONALE: Mutations of the orphan transporter ABCC6 (ATP-binding cassette, subfamily C, member 6) cause the connective tissue disorder pseudoxanthoma elasticum. ABCC6 was thought to be located on the plasma membrane …

Perspectives on: SGP symposium on mitochondrial physiology and medicine: mitochondrial proteome design: from molecular identity to pathophysiological regulation.

Zhang, Jun , Lin, Amanda , Powers, Jared , Lam, Maggie P , Lotz, Christopher …

Journal Article J Gen Physiol Vol. 139 (6 ) pp. 395-406 (2012)

Substrate- and isoform-specific proteome stability in normal and stressed cardiac mitochondria.

Lau, Edward , Wang, Ding , Zhang, Jun , Yu, Hongxiu , Lam, Maggie P …

Journal Article Circ Res Vol. 110 (9 ) pp. 1174-8 (2012)

RATIONALE: Mitochondrial protein homeostasis is an essential component of the functions and oxidative stress responses of the heart. OBJECTIVE: To determine the specificity and efficiency of proteome turnover of the …