Publications

Splice-Junction-Based Mapping of Alternative Isoforms in the Human Proteome.

Lau, Edward , Han, Yu , Williams, Damon R , Thomas, Cody T , Shrestha, …

Journal Article Cell Rep Vol. 29 (11 ) pp. 3751-3765.e5 (2019)

The protein-level translational status and function of many alternative splicing events remain poorly understood. We use an RNA sequencing (RNA- seq)-guided proteomics method to identify protein alternative splicing isoforms in …

Activation of PDGF pathway links LMNA mutation to dilated cardiomyopathy.

Lee, Jaecheol , Termglinchan, Vittavat , Diecke, Sebastian , Itzhaki, Ilanit , Lam, Chi Keung …

Journal Article Nature Vol. 572 (7769 ) pp. 335-340 (2019)

Lamin A/C (LMNA) is one of the most frequently mutated genes associated with dilated cardiomyopathy (DCM). DCM related to mutations in LMNA is a common inherited cardiomyopathy that is associated …

A Premature Termination Codon Mutation in MYBPC3 Causes Hypertrophic Cardiomyopathy via Chronic Activation of Nonsense-Mediated Decay.

Seeger, Timon , Shrestha, Rajani , Lam, Chi Keung , Chen, Caressa , McKeithan, Wesley …

Journal Article Circulation Vol. 139 (6 ) pp. 799-811 (2019)

BACKGROUND: Hypertrophic cardiomyopathy (HCM) is frequently caused by mutations in myosin-binding protein C3 ( MYBPC3) resulting in a premature termination codon (PTC). The underlying mechanisms of how PTC mutations in …

Systems-Wide Approaches in Induced Pluripotent Stem Cell Models.

Lau, Edward , Paik, David T , Wu, Joseph C

Journal Article Annu Rev Pathol Vol. 14 pp. 395-419 (2019)

Human induced pluripotent stem cells (iPSCs) provide a renewable supply of patient-specific and tissue-specific cells for cellular and molecular studies of disease mechanisms. Combined with advances in various omics technologies, …

Identifying High-Priority Proteins Across the Human Diseasome Using Semantic Similarity.

Lau, Edward , Venkatraman, Vidya , Thomas, Cody T , Wu, Joseph C , Van …

Journal Article J Proteome Res Vol. 17 (12 ) pp. 4267-4278 (2018)

Identifying the genes and proteins associated with a biological process or disease is a central goal of the biomedical research enterprise. However, relatively few systematic approaches are available that provide …

Omics, Big Data, and Precision Medicine in Cardiovascular Sciences.

Lau, Edward , Wu, Joseph C

Journal Article Circ Res Vol. 122 (9 ) pp. 1165-1168 (2018)

Prolonged survival of transplanted stem cells after ischaemic injury via the slow release of pro-survival peptides from a collagen matrix.

Lee, Andrew S , Inayathullah, Mohammed , Lijkwan, Maarten A , Zhao, Xin , Sun, …

Journal Article Nat Biomed Eng Vol. 2 (2 ) pp. 104-113 (2018)

Stem-cell-based therapies hold considerable promise for regenerative medicine. However, acute donor-cell death within several weeks after cell delivery remains a critical hurdle for clinical translation. Co- transplantation of stem cells …

NextGen VOICES: Research resolutions.

Olmeta-Schult, Felicia , Segal, Lauren Massa , Tyner, Sam , Moon, Twila Alexandra , Chow, …

Journal Article Science Vol. 359 (6371 ) pp. 26-28 (2018)

Integrated omics dissection of proteome dynamics during cardiac remodeling.

Lau, Edward , Cao, Quan , Lam, Maggie P Y , Wang, Jie , Ng, …

Journal Article Nat Commun Vol. 9 (1 ) pp. 120 (2018)

Transcript abundance and protein abundance show modest correlation in many biological models, but how this impacts disease signature discovery in omics experiments is rarely explored. Here we report an integrated …

Cardiovascular proteomics in the era of big data: experimental and computational advances.

Lam, Maggie P Y , Lau, Edward , Ng, Dominic C M , Wang, Ding …

Review Article Clin Proteomics Vol. 13 pp. 23 (2016)

Proteomics plays an increasingly important role in our quest to understand cardiovascular biology. Fueled by analytical and computational advances in the past decade, proteomics applications can now go beyond merely …

Data-Driven Approach To Determine Popular Proteins for Targeted Proteomics Translation of Six Organ Systems.

Lam, Maggie P Y , Venkatraman, Vidya , Xing, Yi , Lau, Edward , Cao, …

Journal Article J Proteome Res Vol. 15 (11 ) pp. 4126-4134 (2016)

Amidst the proteomes of human tissues lie subsets of proteins that are closely involved in conserved pathophysiological processes. Much of biomedical research concerns interrogating disease signature proteins and defining their …

Connecting the Dots: From Big Data to Healthy Heart.

Lau, Edward , Watson, Karol E , Ping, Peipei

Journal Article Circulation Vol. 134 (5 ) pp. 362-4 (2016)

Identifying Low-Abundance Biomarkers: Aptamer-Based Proteomics Potentially Enables More Sensitive Detection in Cardiovascular Diseases.

Gramolini, Anthony , Lau, Edward , Liu, Peter P

Journal Article Circulation Vol. 134 (4 ) pp. 286-9 (2016)

A large dataset of protein dynamics in the mammalian heart proteome.

Lau, Edward , Cao, Quan , Ng, Dominic C M , Bleakley, Brian J , …

Journal Article Sci Data Vol. 3 pp. 160015 (2016)

Protein stability is a major regulatory principle of protein function and cellular homeostasis. Despite limited understanding on mechanisms, disruption of protein turnover is widely implicated in diverse pathologies from heart …

Label-Free Quantitative Proteomics Reveals Survival Mechanisms Developed by Hypertrophic Chondrocytes under ER Stress.

Kudelko, Mateusz , Chan, Cecilia W L , Sharma, Rakesh , Yao, Qing , Lau, …

Journal Article J Proteome Res Vol. 15 (1 ) pp. 86-99 (2016)

Emerging evidence implicates ER stress caused by unfolded mutant proteins in chondrocytes as the underlying pathology of chondrodysplasias. ER stress is triggered in hypertrophic chondrocytes (HCs) in a mouse model …

Prioritizing Proteomics Assay Development for Clinical Translation.

Lam, Maggie P Y , Venkatraman, Vidya , Cao, Quan , Wang, Ding , Dincer, …

Journal Article J Am Coll Cardiol Vol. 66 (2 ) pp. 202-4 (2015)

Spatial and temporal dynamics of the cardiac mitochondrial proteome.

Lau, Edward , Huang, Derrick , Cao, Quan , Dincer, T Umut , Black, Caitie …

Journal Article Expert Rev Proteomics Vol. 12 (2 ) pp. 133-46 (2015)

Mitochondrial proteins alter in their composition and quantity drastically through time and space in correspondence to changing energy demands and cellular signaling events. The integrity and permutations of this dynamism …

Mitochondrial protein turnover: methods to measure turnover rates on a large scale.

Chan, X'avia C Y , Black, Caitlin M , Lin, Amanda J , Ping, Peipei …

Journal Article J Mol Cell Cardiol Vol. 78 pp. 54-61 (2015)

Mitochondrial proteins carry out diverse cellular functions including ATP synthesis, ion homeostasis, cell death signaling, and fatty acid metabolism and biogenesis. Compromised mitochondrial quality control is implicated in various human …

Characterization of human plasma proteome dynamics using deuterium oxide.

Wang, Ding , Liem, David A , Lau, Edward , Ng, Dominic C M , …

Journal Article Proteomics Clin Appl Vol. 8 (7-8 ) pp. 610-9 (2014)

PURPOSE: High-throughput quantification of human protein turnover via in vivo administration of deuterium oxide ((2) H2 O) is a powerful new approach to examine potential disease mechanisms. Its immediate clinical …

Lysine ubiquitination and acetylation of human cardiac 20S proteasomes.

Zong, Nobel , Ping, Peipei , Lau, Edward , Choi, Howard Jh , Ng, Dominic …

Journal Article Proteomics Clin Appl Vol. 8 (7-8 ) pp. 590-594 (2014)

PURPOSE: Altered proteasome functions are associated with multiple cardiomyopathies. While the proteasome targets polyubiquitinated proteins for destruction, it itself is modifiable by ubiquitination. We aim to identify the exact ubiquitination …